13817140470
当前位置:上海劲马实验设备有限公司 > 产品中心 > ELISA试剂盒 > 小鼠ELISA试剂盒 > 小鼠T细胞活化连接蛋白(LAT)ELISA试剂盒
上海劲马生物销售各种ELISA试剂盒,有RD,RB原装,标本齐全,提供代测。如有疑问,可与上海劲马生物销售或技术联系 负责人:王经理。
产品名称:小鼠T细胞活化连接蛋白(LAT)ELISA试剂盒
英文名称:Mouse Linker for activation of T cell,LAT ELISA KIT
产品规格:96人份/48人份
各种种属:人、大小鼠、豚鼠、兔子、猪、犬、牛、羊…
标本齐全:血清、血浆、细胞培养上清液、组织匀浆、组织液、关节液、胸腔溢液等…
保存条件:2-8℃
联系电话:021-6051 7348,138 1714 0470
提供ELISA代做实验 在我司购买试剂盒提供免费代测
Assay procedure
1. Dilute and add sample:Dilute Original density Standard as follow table:
160ng/L | 5 Standard | 150μl Original density Standard+150μl Standard diluent |
80ng/L | 4 Standard | 150μl 5 Standard+150μl Standard diluent |
40ng/L | 3 Standard | 150μl 4 Standard+150μl Standard diluent |
20ng/L | 2 Standard | 150μl 3 Standard +150μl Standard diluent |
10ng/L | 1 Standard | 150μl 2 Standard +150μl Standard diluent |
2.add sample:Set blank wells separately (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
4.Configurate liquid: 30-fold(or 20-fold) wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.
5.washing:Uncover Closure plate membrane, discardLiquid, dry by swing, add washing buffer to every well, still for 30s then drain,repeat 5 times, dry by pat.
6.add enzyme:Add HRP-Conjugate reagent 50μl to each well, except blank well.
7.incubate:Operation with 3.
8.washing:Operation with 5.
9.color:Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃
10.Stop the reaction:Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).
11.assay:take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
“小鼠T细胞活化连接蛋白(LAT)ELISA试剂盒”含税含运费,提供代测,中英文双版说明书,品牌美国RD、RB、IBL、Omega,质量,详情请来电向我司索取信息。
更新时间:2024/2/19 16:56:04
人ELISA试剂盒 | 大鼠ELISA试剂盒 | 小鼠ELISA试剂盒 | 兔ELISA试剂盒 | 豚鼠ELISA试剂盒 | 猴ELISA试剂盒 | 绵羊ELISA试剂盒 | 鸡ELISA试剂盒 | 猪ELISA试剂盒 | 鸭ELISA试剂盒 | 马ELISA试剂盒 | 骆驼ELISA试剂盒 | 山羊ELISA试剂盒 | 植物ELISA试剂盒 | 牛ELISA试剂盒 | 犬ELISA试剂盒 | 鱼类ELISA试剂盒 | 鹿ELISA试剂盒 |
阿仪网 设计制作,未经允许翻录必究. 联系人:冯经理 联系电话:13817140470 ICP备案号:沪ICP备15015681号-6 总访问量:12384576
主营产品:ELISA试剂盒,猪ELISA试剂盒,鸡ELISA试剂盒,绵羊ELISA试剂盒,猴ELISA试剂盒,动物试剂盒、生物试剂、对照品、抗体、血清、培养基等